Composite

Part:BBa_K2486019:Design

Designed by: Cauă Westmann, Gabriel Lencioni Lovate   Group: iGEM17_USP-Brazil   (2017-10-24)


PlldR_tetR/LldR based lactate biosensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 874
    Illegal NheI site found at 897
    Illegal NheI site found at 1033
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 284
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1743


Design Notes

working with gblocks was a little bit problematic


Source

3 a assembly of gblocks

References